I want to request testing by your laboratory, what do I have to do for this?

You will need to contact your doctor. Please tell your doctor to visit our website and to download our test kit order form (USA or outside USA), fill and sign it and submit it to our office together with a copy of his/her medical license. After that we will send a test kit to your doctor’s office.

I am a doctor and it is the first time I am going to send you samples. Should I send you my medical license?

Yes, please submit a copy of your medical license to our office by fax or e-mail.

The instruction sheet for Nagalase says to stop immune stimulants prior to testing. However, these are part of the treatment for the patient. Will these immune stimulants affect the result if they are not stopped for testing?

The immune stimulants decrease the Nagalase activity. We advise to draw blood for the Nagalase test just before the immune stimulant medication is given. If this medication is given once a week, as is mostly the case, then this will pose no problem. However, if a one week interval is not possible: at least try to use the same time period each time to have the best follow up.

The instruction sheet for the Methylation Pathway Panel says to stop medication (if possible) prior to testing. What kind of medication should be discontinued and for what time?

It would be better to stop intake of vitamin B6 and B12 for 48 hours before the blood draw since the high intake of these vitamins may affect the results.

Kryptopyrrole Test | Health Diagnostics And Research Institute

Kryptopyrrole

Urinary Test for Pyroluria

Pyroluria is caused by an inborn error in synthesizing and metabolizing hemoglobin resulting in elevated levels of hydroxyhemopyrrolin-2-one (HPL) as well as kryptopyrrole (KPU, 2,4 dimethyl-3-ethyl pyrrole), which are excreted in the urine.

As these pyrroles bind to – and thus deplete – zinc, vitamin B6, and omega 6, pyroluria is associated with deficiencies of these nutrients as well as a deficiency of serotonin, a neurotransmitter whose synthesis requires vitamin B6. Elevated levels of pyrroles also cause a variety of behavioral, neurological, and cognitive symptoms, including irritability, poor stress control, mood swings, impaired short term memory, and depression. Higher concentrations of pyrroles have been found in the urine of patients with autism, learning disabilities, alcoholism, ADHD, depression, bipolar disorders, Down Syndrome, schizophrenia, and possibly chronic Lyme disease. Both KPU and HPL are known to be associated with abdominal pain, acute intermittent porphyria (AIP), and/or haemolytic crisis.

The urinary KPU test can confirm pyroluria and thereby facilitate appropriate diagnosis and treatment, generally involving supplementation with zinc and vitamin B6.

Interpretation of random KPU results

< 15 µg/dL:
15 – 25 µg/dL:
> 25 µg/dL:
> 50 µg/dL:

negative
elevated
positive
strongly positive

(no evidence of pyroluria)
(may benefit from treatment)
(treatment recommended)
(treatment strongly recommended)

References

McGinnis W, Audhya T, Walsh W et al. Discerning the Mauve factor, Part I. Alternative Therapies in Health and Medicine, 14:40-50, 2008.
McGinnis W, Audhya T, Walsh W et al. Discerning the Mauve factor, Part II. Alternative Therapies in Health and Medicine, 14:56-62, 2008.